When pipetting small volumes, accuracy and precision are ensured by using the proper technique and tools. The following techniques are recommended for pipetting small volumes:
- Change pipette tips frequently: Pipette tipsshould be changed often, particularly when pipetting small volumes. This is due to the possibility that minute liquid particles will stick to the inside of the tip, reducing the accuracy of subsequent pipetting.
- Use low-retention pipette tips: When pipetting small volumes, it's crucial to take liquid retention into account. Low-retention pipette tips are designed with this in mind. Usually composed of a different type of polypropylene, these tips are lubricated with silicone to lower surface tension and stop liquid from sticking to the inside of the tip.
- Use the correct pipette: Select a pipette made especially for pipetting tiny amounts of liquid. Compared to pipettes made for larger volumes, these pipettes have finer graduations and smaller plunger sizes.
- Pause before dispensing: Before dispensing, allow the liquid to settle while you aspirate it. By doing this, you can make sure you're dispensing the right amount by allowing the liquid to settle in the tip.
- Use a slow pipetting speed: When dispensing small volumes, use the pipette slowly. This will decrease the possibility of air bubbles forming by allowing the liquid more time to flow into and out of the tip.
- Calibrate your pipette: You can make sure that the right amount of liquid is being dispensed by regularly calibrating your pipette. This is particularly crucial for pipetting tiny volumes.
- Pre-wet the pipette tip: You can reduce liquid retention and increase accuracy by lightly moistening the pipette tip with a small amount of the liquid you plan to pipette. This is particularly crucial for liquids that are sticky or viscous.
- Dispense at an angle: A 45-degree angle should be used to dispense the liquid onto the vessel's surface. By doing this, you can lessen the chance that the liquid will spill or fall off the side of the container.
- Touch the pipette tip to the side of the vessel: To get rid of any leftover liquid after the liquid has been dispensed, touch the pipette's tip to the vessel's side. If you do this, you can be sure that the right amount is being dispensed.
- Practice proper technique: Use the appropriate pipetting technique to guarantee accurate and consistent results. This entails using the pipette properly, applying steady pressure to the plunger, and avoiding sudden movements.
You can increase the precision and accuracy of pipetting small volumes and guarantee consistent and repeatable results in your laboratory tests by adhering to these best practices.
